Bi-Enzymatic Conductometric Biosensor for Detection of Heavy Metal Ions and Pesticides in Water Samples Based on Enzymatic Inhibition in Arthrospira platensis - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Environmental Protection Année : 2014

Bi-Enzymatic Conductometric Biosensor for Detection of Heavy Metal Ions and Pesticides in Water Samples Based on Enzymatic Inhibition in Arthrospira platensis

Biocapteur bimézymétrique conductimétrique pour la détection d'ions des métaux lourds et des pesticides dans des les eaux, basés sur l'inhibition enzymatique d'Arthrospira platensis

Résumé

An original bi-enzymatic biosensor was designed by immobilizing Arthrospira platensis cells, called Spirulina, on gold interdigitated transducers. Phosphatase and esterase activities were inhibited, respectively, by heavy metals and by pesticides. Inhibition activities were observed with different mixtures of pesticides + heavy metals. The quantification limits for Cd2+ and Hg2+ are 10−20 M in mixture and in pure solution. The quantification limits of parathion-methyl, paraoxon-methyl and triazine are respectively 10−20 M, 10−18 M and 10−20 M in mixture and pure solutions. These results show that there is no synergistic effect between the two families of pollutants. Qualitative contamination of effluent samples and their purification after passing through a municipal wastewater treatment plant were observed by our bi-enzymatic biosensor and confirmed by classical analytical techniques for heavy metal ions.
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Dates et versions

hal-01529622 , version 1 (31-05-2017)

Identifiants

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Nadèje Tekaya, Olga Saiapina, Hatem Ben Ouada, Florence Lagarde, Philippe Namour, et al.. Bi-Enzymatic Conductometric Biosensor for Detection of Heavy Metal Ions and Pesticides in Water Samples Based on Enzymatic Inhibition in Arthrospira platensis. Journal of Environmental Protection, 2014, 5, pp.441-453. ⟨10.4236/jep.2014.55047⟩. ⟨hal-01529622⟩
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