A global benchmark study using affinity-based biosensors
Rebecca L. Rich
(1)
,
Giuseppe A. Papalia
(1)
,
Peter J. Flynn
(2)
,
Jamie Furneisen
(3)
,
John Quinn
(4)
,
Joshua S. Klein
(5)
,
Phini S. Katsamba
(6)
,
M. Brent Waddell
(7)
,
Michael Scott
(8)
,
Joshua Thompson
(9)
,
Judie Berlier
(9)
,
Schuyler Corry
(9)
,
Mireille Baltzinger
(10)
,
Gabrielle Zeder-Lutzi
(10)
,
Andreas Schoenemann
(11)
,
Anca Clabbers
(12)
,
Sébastien Wieckowski
(13)
,
Mary M. Murphy
(14)
,
Phillip Page
(14)
,
Thomas E. Ryan
(14)
,
Jay Duffner
(15)
,
Tanmoy Ganguly
(15)
,
John Corbin
(16)
,
Satyen Gautam
(17)
,
Gregor Anderluh
(18)
,
Andrej Bavdek
(18)
,
Dana Reichmann
(19)
,
Satya P. Yadav
(20)
,
Eric Hommema
(21)
,
Ewa Pol
(22)
,
Andrew Drake
(23)
,
Scott Klakamp
(23)
,
Trevor Chapman
(24)
,
Dawn Kernaghan
(25)
,
Ken Miller
(25)
,
Jason Schuman
(26)
,
Kevin Lindquist
(26)
,
Kara Herlihy
(26)
,
Michael B. Murphy
(26)
,
Richard Bohnsack
(27)
,
Bruce Andrien
(28)
,
Pietro Brandani
(29)
,
Danny Terwey
(30)
,
Rohn Millican
(31)
,
Ryan J. Darling
(31)
,
Liann Wang
(31)
,
Quincy Carter
(31)
,
Joe Dotzlaf
(31)
,
Jacinto Lopez-Sagaseta
(32)
,
Islay Campbell
(33)
,
Paola Torreri
(34)
,
Sylviane Hoos
(35)
,
Patrick England
(35)
,
Yang Liu
(36)
,
Yasmina Abdiche
(37)
,
Daniel Malashock
(37)
,
Alanna Pinkerton
(37)
,
Melanie Wong
(38)
,
Eileen Lafer
(39)
,
Cynthia Hinck
(39)
,
Kevin Thompson
(40)
,
Carmelo Di Primo
(41)
,
Alison Joyce
(42)
,
Jonathan Brooks
(42)
,
Federico Torta
(43)
,
Anne Birgitte Bagge Hagel
(44)
,
Janus Krarup
(44)
,
Jesper Pass
(44)
,
Monica Ferreira
(45)
,
Sergei Shikov
(46)
,
Malgorzata Mikolajczyk
(47)
,
Yuki Abe
(48)
,
Gaetano Barbato
(49)
,
Anthony M. Giannetti
(50)
,
Ganeshram Krishnamoorthy
(51)
,
Bianca Beusink
(51)
,
Daulet Satpaev
(52)
,
Tiffany Tsang
(53)
,
Eric Fang
(53)
,
James Partridge
(54)
,
Stephen Brohawn
(54)
,
James Horn
(55)
,
Otto Pritsch
(56)
,
Gonzalo Obal
(56)
,
Sanjay Nilapwar
(57)
,
Ben Busby
(58)
,
Gerardo Gutierrez-Sanchez
(59)
,
Ruchira das Gupta
(60)
,
Sylvie Canepa
(61)
,
Krista Witte
(62)
,
Zaneta Nikolovska-Coleska
(63)
,
Yun Hee Cho
(64)
,
Roberta d'Agata
(65)
,
Kristian Schlick
(66)
,
Rosy Calvert
(67)
,
Eva M. Munoz
(68)
,
Maria Jose Hernaiz
(68)
,
Tsafir Bravman
(69)
,
Monica Dines
(69)
,
Min-Hsiang Yang
(70)
,
Agnes Puskas
(71)
,
Erica Boni
(72)
,
Jiejin Li
(73)
,
Martin Wear
(74)
,
Asya Grinberg
(75)
,
Jason Baardsnes
(76)
,
Olan Dolezal
(77)
,
Melicia Gainey
(78)
,
Henrik Anderson
(79)
,
Jinlin Peng
(80)
,
Mark Lewis
(80)
,
Peter Spies
(81)
,
Quyhn Trinh
(82)
,
Sergei Bibikov
(82)
,
Jill Raymond
(82)
,
Mohammed Yousef
(82)
,
Vidya Chandrasekaran
(82)
,
Yuguo Feng
(82)
,
Anne Emerick
(82)
,
Suparna Mundodo
(82)
,
Rejane Guimaraes
(82)
,
Katy Mcgirr
(82)
,
Yue-Ji Li
(83)
,
Heather Hughes
(84)
,
Hubert Mantz
(85)
,
Rostislav Skrabana
(86)
,
Mark Witmer
(87)
,
Joshua Ballard
(88)
,
Loïc M Martin
(89)
,
Petr Skladal
(90)
,
George Korza
(91)
,
Ite Laird-Offringa
(92)
,
Charlene S. Lee
(92)
,
Abdelkrim Khadir
(93)
,
Frank Podlaski
(94)
,
Phillippe Neuner
(95)
,
Julie Rothacker
(96)
,
Ashique Rafique
(97)
,
Nico Dankbar
(98)
,
Peter Kainz
(99)
,
Erk Gedig
(100)
,
Momchilo Vuyisich
(101)
,
Christina Boozer
(102)
,
Nguyen Ly
(103)
,
Mark Toews
(104)
,
Aykut Uren
(105)
,
Oleksandr Kalyuzhniy
(106)
,
Kenneth Lewis
(107)
,
Eugene Chomey
(108)
,
Brian J. Pak
(109)
,
David G. Myszka
(1)
1
School of Medicine [University of Utah, Salt Lake City]
2 KaloBios Pharmaceuticals [San Francisco]
3 Schering–Plough Biopharma [Palo Alto]
4 Nomadics [Oklahoma City]
5 Biochemistry and Molecular Biophysics [Pasadena]
6 Department of Biochemistry and Molecular Biophysics
7 Hartwell Center for Bioinformatics and Biotechnology [Memphis]
8 UZH - Universität Zürich [Zürich] = University of Zurich
9 Molecular Probes/Invitrogen [Eugene]
10 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
11 MerckKGaA
12 Abbott Bioresearch Center [Worcester]
13 IBMC - Institut de biologie moléculaire et cellulaire
14 Reichert [Depew}
15 Momenta Pharmaceuticals [Cambridge]
16 XOMA (U.S.)
17 Department of Chemical and Biomolecular Engineering, National University of Singapore
18 Department of Biology [Ljubljiana]
19 Weizmann Institute of Science - Department of Biological Chemistry
20 Molecular Biotechnology Core Laboratory [Cleveland]
21 ThermoFisher Scientific [Rockford]
22 Biacore/GE Healthcare [Uppsala]
23 AstraZeneca [Hayward]
24 Neurodegeneration Research Department [Harlow]
25 MedImmune
26 Biacore/GE Healthcare [Piscataway]
27 Department of Biochemistry [Milwaukee]
28 Alexion Pharmaceuticals [Cheshire]
29 Applied Biosystems [Foster City]
30 diaDexus [South San Francisco]
31 Eli Lilly and Company [Indianapolis]
32 CIMA - Center for Applied Medical Research [Plamplona]
33 EMD Lexigen Research Center [Billerica]
34 Department of Cell Biology and Neuroscience [Rome]
35 Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry
36 Department of Chemistry [Atlanta]
37 Pfizer/Rinat Laboratories [South San Francisco]
38 PDLBioPharma [Fremont]
39 Department of Biochemistry [San Antonio]
40 Akubio [Cambridge]
41 ARN : régulations naturelle et artificielle
42 Wyeth Research [Cambridge]
43 Department of Biochemistry and Molecular Biology [Odense]
44 NovoNordisk [Gentofte]
45 Södertörns University College [Huddinge]
46 Department of Biochemistry [Philadelphia]
47 FDA - U.S. Food and Drug Administration
48 Department of Biochemical Engineering [London]
49 Merck [Rome]
50 Roche [Palo Alto]
51 University of Twente
52 Agensys [Santa Monica]
53 Novartis [Emeryville]
54 MIT - Massachusetts Institute of Technology
55 Northern Illinois University
56 Institut Pasteur de Montevideo
57 University of Manchester [Manchester]
58 Department of Physiology [Baltimore]
59 Complex Carbohydrate Research Center [Athens, GA, USA]
60 Adnexus Therapeutics [Waltham}
61 PRC - Physiologie de la reproduction et des comportements [Nouzilly]
62 ForteBio [Menlo Park]
63 University of Michigan [Ann Arbor]
64 Human Genome Sciences [Rockville]
65 Department of Chemical Sciences [Catania]
66 MSU - Montana State University
67 GKT School of Biomedical Sciences [London]
68 Organic and Pharmaceutical Chemistry Department [Madrid]
69 Bio-Rad Haifa
70 Institute of Chemistry [Taipei]
71 Department of Microbiology and Molecular Genetics
72 FHCRC - Fred Hutchinson Cancer Research Center [Seattle]
73 Division of Molecular Structure [London]
74 Centre for Translational and Chemical Biology, Institute of Structural and Molecular Biology [Edinburgh]
75 Acceleron Pharma [Cambridge]
76 Biotechnology Research Institute [Montreal]
77 CSIRO Health Sciences and Nutrition [Parkville]
78 Battelle Biomedical Research Center [Columbus]
79 Attana AB [Stockholm]
80 Corning Incorporated
81 School of Life Sciences, Institute for Chemistry and Bioanalytics [Muttenz]
82 Bio-Rad [Hercules]
83 Monsanto [Chesterfield]
84 Genzyme [Cambridge]
85 Universität des Saarlandes [Saarbrücken] = Saarland University [Saarbrücken]
86 Institute of Neuroimmunology of SAS [Bratislava]
87 Bristol-Myers Squibb [Princeton]
88 Array Biopharma [Boulder]
89 SIMoS - Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO)
90 MUNI - Masaryk University [Brno]
91 UCONN - University of Connecticut
92 USC - University of Southern California
93 GeminX Biotechnologies [Montreal]
94 Hoffman–La Roche [Nutley]
95 IRBM, Pomezia [Rome]
96 Ludwig Institute for Cancer Research
97 Regeneron Pharmaceuticals [Tarrytown, NY]
98 University of Muenster
99 Department of Molecular Biology [Salzburg]
100 XanTec Bioanalytics [Muenster]
101 Biosciences Division [Los Alamos]
102 Lumera [Bothell]
103 ASU - Arizona State University [Tempe]
104 Dyax [Cambridge]
105 Lombardi Comprehensive Cancer Center
106 Department of Biochemistry [Seattle]
107 ZymoGenetics
108 Bio-Rad Canada [Edmonton]
109 Bio-Rad Canada [Toronto]
2 KaloBios Pharmaceuticals [San Francisco]
3 Schering–Plough Biopharma [Palo Alto]
4 Nomadics [Oklahoma City]
5 Biochemistry and Molecular Biophysics [Pasadena]
6 Department of Biochemistry and Molecular Biophysics
7 Hartwell Center for Bioinformatics and Biotechnology [Memphis]
8 UZH - Universität Zürich [Zürich] = University of Zurich
9 Molecular Probes/Invitrogen [Eugene]
10 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
11 MerckKGaA
12 Abbott Bioresearch Center [Worcester]
13 IBMC - Institut de biologie moléculaire et cellulaire
14 Reichert [Depew}
15 Momenta Pharmaceuticals [Cambridge]
16 XOMA (U.S.)
17 Department of Chemical and Biomolecular Engineering, National University of Singapore
18 Department of Biology [Ljubljiana]
19 Weizmann Institute of Science - Department of Biological Chemistry
20 Molecular Biotechnology Core Laboratory [Cleveland]
21 ThermoFisher Scientific [Rockford]
22 Biacore/GE Healthcare [Uppsala]
23 AstraZeneca [Hayward]
24 Neurodegeneration Research Department [Harlow]
25 MedImmune
26 Biacore/GE Healthcare [Piscataway]
27 Department of Biochemistry [Milwaukee]
28 Alexion Pharmaceuticals [Cheshire]
29 Applied Biosystems [Foster City]
30 diaDexus [South San Francisco]
31 Eli Lilly and Company [Indianapolis]
32 CIMA - Center for Applied Medical Research [Plamplona]
33 EMD Lexigen Research Center [Billerica]
34 Department of Cell Biology and Neuroscience [Rome]
35 Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry
36 Department of Chemistry [Atlanta]
37 Pfizer/Rinat Laboratories [South San Francisco]
38 PDLBioPharma [Fremont]
39 Department of Biochemistry [San Antonio]
40 Akubio [Cambridge]
41 ARN : régulations naturelle et artificielle
42 Wyeth Research [Cambridge]
43 Department of Biochemistry and Molecular Biology [Odense]
44 NovoNordisk [Gentofte]
45 Södertörns University College [Huddinge]
46 Department of Biochemistry [Philadelphia]
47 FDA - U.S. Food and Drug Administration
48 Department of Biochemical Engineering [London]
49 Merck [Rome]
50 Roche [Palo Alto]
51 University of Twente
52 Agensys [Santa Monica]
53 Novartis [Emeryville]
54 MIT - Massachusetts Institute of Technology
55 Northern Illinois University
56 Institut Pasteur de Montevideo
57 University of Manchester [Manchester]
58 Department of Physiology [Baltimore]
59 Complex Carbohydrate Research Center [Athens, GA, USA]
60 Adnexus Therapeutics [Waltham}
61 PRC - Physiologie de la reproduction et des comportements [Nouzilly]
62 ForteBio [Menlo Park]
63 University of Michigan [Ann Arbor]
64 Human Genome Sciences [Rockville]
65 Department of Chemical Sciences [Catania]
66 MSU - Montana State University
67 GKT School of Biomedical Sciences [London]
68 Organic and Pharmaceutical Chemistry Department [Madrid]
69 Bio-Rad Haifa
70 Institute of Chemistry [Taipei]
71 Department of Microbiology and Molecular Genetics
72 FHCRC - Fred Hutchinson Cancer Research Center [Seattle]
73 Division of Molecular Structure [London]
74 Centre for Translational and Chemical Biology, Institute of Structural and Molecular Biology [Edinburgh]
75 Acceleron Pharma [Cambridge]
76 Biotechnology Research Institute [Montreal]
77 CSIRO Health Sciences and Nutrition [Parkville]
78 Battelle Biomedical Research Center [Columbus]
79 Attana AB [Stockholm]
80 Corning Incorporated
81 School of Life Sciences, Institute for Chemistry and Bioanalytics [Muttenz]
82 Bio-Rad [Hercules]
83 Monsanto [Chesterfield]
84 Genzyme [Cambridge]
85 Universität des Saarlandes [Saarbrücken] = Saarland University [Saarbrücken]
86 Institute of Neuroimmunology of SAS [Bratislava]
87 Bristol-Myers Squibb [Princeton]
88 Array Biopharma [Boulder]
89 SIMoS - Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO)
90 MUNI - Masaryk University [Brno]
91 UCONN - University of Connecticut
92 USC - University of Southern California
93 GeminX Biotechnologies [Montreal]
94 Hoffman–La Roche [Nutley]
95 IRBM, Pomezia [Rome]
96 Ludwig Institute for Cancer Research
97 Regeneron Pharmaceuticals [Tarrytown, NY]
98 University of Muenster
99 Department of Molecular Biology [Salzburg]
100 XanTec Bioanalytics [Muenster]
101 Biosciences Division [Los Alamos]
102 Lumera [Bothell]
103 ASU - Arizona State University [Tempe]
104 Dyax [Cambridge]
105 Lombardi Comprehensive Cancer Center
106 Department of Biochemistry [Seattle]
107 ZymoGenetics
108 Bio-Rad Canada [Edmonton]
109 Bio-Rad Canada [Toronto]
Sylviane Hoos
- Fonction : Auteur
- PersonId : 747020
- IdHAL : sylviane-hoos
- ORCID : 0000-0002-4029-562X
Patrick England
- Fonction : Auteur
- PersonId : 735030
- IdHAL : patrick-england
- ORCID : 0000-0001-6410-5918
Carmelo Di Primo
- Fonction : Auteur
- PersonId : 772950
- ORCID : 0000-0002-0509-8399
- IdRef : 071086579
Loïc M Martin
- Fonction : Auteur
- PersonId : 740796
- IdHAL : loic-mjc-martin
- ORCID : 0000-0002-7940-2955
- IdRef : 169807029
Résumé
To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used. (C) 2008 Elsevier Inc. All rights reserved.
Domaines
Médecine humaine et pathologie| Origine | Fichiers produits par l'(les) auteur(s) |
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