Observation of correlated X-ray scattering at atomic resolution - Archive ouverte HAL
Article Dans Une Revue Philosophical Transactions of the Royal Society B: Biological Sciences Année : 2014

Observation of correlated X-ray scattering at atomic resolution

Derek Mendez
  • Fonction : Auteur
Thomas J. Lane
  • Fonction : Auteur
Jonas Sellberg
  • Fonction : Auteur
Herschel Watkins
  • Fonction : Auteur
Aina E. Cohen
  • Fonction : Auteur
Michael Soltis
  • Fonction : Auteur
James Spudich
  • Fonction : Auteur
Vijay Pande
  • Fonction : Auteur
Daniel Ratner
  • Fonction : Auteur
Sebastian Doniach
  • Fonction : Auteur

Résumé

Tools to study disordered systems with local structural order, such as proteins in solution, remain limited. Such understanding is essential for e.g. rational drug design. Correlated X-ray scattering (CXS) has recently attracted new interest as a way to leverage next-generation light sources to study such disordered matter. The CXS experiment measures angular correlations of the intensity caused by the scattering of X-rays from an ensemble of identical particles, with disordered orientation and position. Averaging over 15 496 snapshot images obtained by exposing a sample of silver nanoparticles in solution to a micro-focused synchrotron radiation beam, we report on experimental efforts to obtain CXS signal from an ensemble in three dimensions. A correlation function was measured at wide angles corresponding to atomic resolution that matches theoretical predictions. These preliminary results suggest that other CXS experiments on disordered ensembles\textemdashsuch as proteins in solution\textemdashmay be feasible in the future.

Dates et versions

hal-01519372 , version 1 (06-05-2017)

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Citer

Derek Mendez, Thomas J. Lane, Jongmin Sung, Jonas Sellberg, Clément Levard, et al.. Observation of correlated X-ray scattering at atomic resolution. Philosophical Transactions of the Royal Society B: Biological Sciences, 2014, 369 (1647), ⟨10.1098/rstb.2013.0315⟩. ⟨hal-01519372⟩
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