Concentration and purification of lycopene from watermelon juice by integrated microfiltration-based processes
Résumé
The aim of the study was to evaluate a new process based on crossflow microfiltration for
concentration and partial purification of lycopene from watermelon. The process included
sequentially juice extraction, enzymatic liquefaction, crossflow microfiltration and
centrifugation. Different technological schemes were compared including or not a
diafiltration step in order to remove soluble solids and increase lycopene purity.
Microfiltration was performed at 50-60 °C using alumina tubular membranes with average
pore diameter from 0.2 to 1.4 m and varying the transmembrane pressure from 0.5 to 2.0
bar. Using the best operating conditions at the crossflow microfiltration step, permeate flux
was close to 110 L.h-1.m-2 and lycopene concentration was increased 11 times in the
retentate. Centrifugation at 10,000 g for 10 min, enabled to enhance lycopene concentration
up to 4 times. Diafiltration allowed to decrease total soluble solids from 100 to 20 g.kg-1
and so to purify lycopene further. No cis-isomerization was highlighted that proved that the
process maintained carotenoid integrity. Finally, the optimal processing scheme allowed to
obtain a natural extract of lycopene 42 times more concentrated and 34 times purer than the initial juice. The results looked very promising for industrial application even if economic
viability has still to be assessed.
Domaines
Sciences du Vivant [q-bio]Origine | Fichiers produits par l'(les) auteur(s) |
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