Evidence of biological activity of Mentha species extracts on apoptotic and autophagic targets on murine RAW264.7 and human U937 monocytic cells - Archive ouverte HAL Access content directly
Journal Articles Pharmaceutical biology Year : 2017

Evidence of biological activity of Mentha species extracts on apoptotic and autophagic targets on murine RAW264.7 and human U937 monocytic cells

Abstract

Context: Mints (Lamiaceae) are used as traditional remedies for the treatment of several diseases. Their extracts are recognized as anti-inflammatory compounds. Objective: This study characterized the cytotoxic effects of Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L). Huds (MR) on macrophage cells (RAW264.7; U937) and determined their impact on apoptosis and autophagy, which can play a role in controlling inflammation. Materials and methods: The extracts were prepared in culture medium and tested from 25 to 400 mu g/mL after 24-48 h of treatment. To show the effect of the aqueous ethanol (50%) extracts on apoptosis and authophagy, the presence of cleaved caspase-3, and the conversion of LC3-I to LC3-II was evaluated by Western blotting. Results: Compared with the MTT assay, crystal violet showed a pronounced decrease in the number of cells with all extracts at 48 h. Calculated IC50 values were 257.31, 207.82 and 368.02 mu g/mL for MS, MP and MR, respectively. A significant increase in PI positive cells was observed with all extracts at 200-400 mu g/mL. Mitochondrial dysfunctions and nuclear morphological changes were detected with MS and MR extracts at 400 mu g/mL. At this concentration, no cleaved caspase-3 was found whereas stabilized caspase-3 in its dimeric form was identified. MS and MR extracts also favour LC3-I to LC3-II conversion which is a criterion of autophagy. Conclusions: The cytotoxic profiles depend on the extracts considered; MS extract showed the strong activity. However, all the mint extracts studied interact with the apoptotic and autophagic pathways at elevated concentrations.
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hal-01513649 , version 1 (08-07-2019)

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Fatiha Brahmi, Samia Hadj-Ahmed, Amira Zarrouk, Maryem Bezine, Thomas Nury, et al.. Evidence of biological activity of Mentha species extracts on apoptotic and autophagic targets on murine RAW264.7 and human U937 monocytic cells. Pharmaceutical biology, 2017, 55 (1), pp.286-293. ⟨10.1080/13880209.2016.1235208⟩. ⟨hal-01513649⟩
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