Effects of perinatal odorization on the development of food preferences in mice: a preliminary study
Résumé
The purpose of the thesis is to evaluate the consequences of the perinatal odorant environmental changes on the plasticity of the olfactory system and associated behaviors in mice strain (MI7-GFP) expressing an olfactory receptor coupled to a GFP reporter, whose preferential ligand is known ( heptanal ; Bozza et al., 2002). We wonder to examine the changes in the olfactory pathways induced by maternal odorization at two levels of the olfactory system, the mucosa and the bulb, using combined approaches from molecular ( immunohistochemistry , electrophysiology, biochemistry, qPCR , DNA methylation ) to behavioral ones (food choices, odorant detection thresholds). In a preliminary set of experiments, we tested different conditions of maternal exposition to heptanal (odorized water or food pellets impregnation at various concentrations) on C57/BL6c mice as a model. We measured the consequences of the odorization on food and water consumption, we checked the presence of the odorant in various biological fluids of mothers by GC/MS and we registered olfactory-driven behaviors related to olfactory maternal cues at postnatal day3 and day12 on odorized pups as compared to controls.
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