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Communication Dans Un Congrès Année : 2017

Scale-up production of vitamin-whey proteins coacervates and their protective property against storage degradations.

Résumé

The production of natural biocarriers for the protection and controlled delivery of bioactives is of great interest to develop natural functional foods, with enhanced health benefits. The feasibility of heteroprotein complex coacervates formed between two whey proteins (WP), Beta-Lactoglobulin (BLG) and Lactoferrin (LF) to efficiently entrap vitamin B9 has been demonstrated. In the present communication, we investigated the bench scale production of such B9 loaded BLG-LF coacervates (B9-WP coacervates) and their protective ability against B9 degradations during storage (freeze-drying, UV light radiations and accelerated H2O2 induced oxidation). Complex coacervation was performed at bench scale, using commercial protein solutions. The efficiency of two mixing systems, batch versus continuous, were compared. In the continuous mixing system, the formation of B9-WP coacervates was found to be flow-dependant (Figure 1). The highest WP coacervation yield was obtained for a minimum flow rate of 200 mL/min. Under optimized conditions, bench scale efficiency similar to that found for laboratory scale was reached with a coacervation yield of 65 % and a B9 entrapment of 98 %, demonstrating an efficient scaling-up. When entrapped into WP coacervates B9 showed higher stability during freeze-drying with a loss of about 3%, compared to 12% obtained for free unentrapped B9. Additionally, WP coacervates showed good protective effect of B9 against UV irradiation and H2O2 induced oxidation. Hence, heteroprotein complex coacervates constitute a promising biocarriers for the protection of bioactives against chemical and physical degradation. For controlled delivery purpose, in vitro and in vivo studies are in progress to evaluate the effectiveness and optimal release of entrapped B9 molecules. The production of natural biocarriers for the protection and controlled delivery of bioactives is of great interest to develop natural functional foods, with enhanced health benefits. The feasibility of heteroprotein complex coacervates formed between two whey proteins (WP), Beta-Lactoglobulin (BLG) and Lactoferrin (LF) to efficiently entrap vitamin B9 has been demonstrated. In the present communication, we investigated the bench scale production of such B9 loaded BLG-LF coacervates (B9-WP coacervates) and their protective ability against B9 degradations during storage (freeze-drying, UV light radiations and accelerated H2O2 induced oxidation). Complex coacervation was performed at bench scale, using commercial protein solutions. The efficiency of two mixing systems, batch versus continuous, were compared. In the continuous mixing system, the formation of B9-WP coacervates was found to be flow-dependant (Figure 1). The highest WP coacervation yield was obtained for a minimum flow rate of 200 mL/min. Under optimized conditions, bench scale efficiency similar to that found for laboratory scale was reached with a coacervation yield of 65 % and a B9 entrapment of 98 %, demonstrating an efficient scaling-up. When entrapped into WP coacervates B9 showed higher stability during freeze-drying with a loss of about 3%, compared to 12% obtained for free unentrapped B9. Additionally, WP coacervates showed good protective effect of B9 against UV irradiation and H2O2 induced oxidation. Hence, heteroprotein complex coacervates constitute a promising biocarriers for the protection of bioactives against chemical and physical degradation. For controlled delivery purpose, in vitro and in vivo studies are in progress to evaluate the effectiveness and optimal release of entrapped B9 molecules.
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Dates et versions

hal-01497614 , version 1 (29-03-2017)

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Identifiants

  • HAL Id : hal-01497614 , version 1
  • PRODINRA : 389670

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Anne Laure Chapeau, Pascaline Hamon, Florence Rousseau, Thomas Croguennec, Denis Poncelet, et al.. Scale-up production of vitamin-whey proteins coacervates and their protective property against storage degradations.. Innovations in Food Science & Technology, May 2017, Munich, Germany. ⟨hal-01497614⟩
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