Bronchial Epithelial Cells from Asthmatic Patients Display Less Functional HLA-G Isoform Expression. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Immunology Année : 2017

Bronchial Epithelial Cells from Asthmatic Patients Display Less Functional HLA-G Isoform Expression.

Résumé

Not all asthmatic patients adequately respond to current available treatments, such as inhaled corticosteroids or omalizumab(®). New treatments will aim to target the bronchial epithelium-immune response interaction using different pathways. HLA-G is involved in immunomodulation and may promote epithelial cell differentiation and proliferation. HLA-G protein has several isoforms generated by alternative splicing that might have differential functionalities. HLA-G protein expression and genetic polymorphisms have been reported to be associated with asthma. Our hypothesis is that bronchial epithelium from asthmatic patients displays less functional HLA-G isoforms. HLA-G transcriptional isoforms were quantified by real-time PCR in human bronchial epithelium cells (HBEC) grown in air-liquid interface culture obtained from five healthy controls (HC), seven patients with mild asthma (MA), and seven patients with severe asthma (SA). They were re-differentiated, and IL-13 exposure was used as a proxy for a pro-inflammatory cytokine. HLA-G protein expression was assessed by western blot analysis. HLA-G allele was typed by direct sequencing. Our results showed that both MA and SA display less functional HLA-G isoforms than HC (p < 0.05); in vitro HBEC re-differentiation from SA displays a particular isoform expression profile compared to MA and HC (p = 0.03); HLA-G*01:06 frequency in MA and SA was significantly higher than in the healthy population (p = 0.03 and p < 0.001, respectively); and IL-13 exposure had no impact on HLA-G expression. Our results support that an impaired expression of HLA-G isoforms in asthmatic patients could contribute to the loss of inflammation control and epithelium structural remodeling. Therefore, HLA-G might be an interesting alternative target for asthmatic patients not adequately responding to current drugs.
Fichier principal
Vignette du fichier
fimmu-08-00006.pdf (1.25 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01492659 , version 1 (12-06-2018)

Licence

Paternité

Identifiants

Citer

Federico Carlini, Christophe Picard, Céline Garulli, David Piquemal, Pierre L. Roubertoux, et al.. Bronchial Epithelial Cells from Asthmatic Patients Display Less Functional HLA-G Isoform Expression.. Frontiers in Immunology, 2017, 8, pp.6. ⟨10.3389/fimmu.2017.00006⟩. ⟨hal-01492659⟩
119 Consultations
60 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More