Combining μ X-ray fluorescence, μXANES and μXRD to shed light on Zn2+ cations in cartilage and meniscus calcifications - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Trace Elements in Medicine and Biology Année : 2013

Combining μ X-ray fluorescence, μXANES and μXRD to shed light on Zn2+ cations in cartilage and meniscus calcifications

Arnaud Dessombz
  • Fonction : Auteur
Christelle Nguyen
  • Fonction : Auteur
Hang-Korng Ea
  • Fonction : Auteur
Stephan Rouzière
  • Fonction : Auteur
Eddy Foy
Didier Hannouche
  • Fonction : Auteur
Solène Reguer
  • Fonction : Auteur
Frederic-Emmanuel Picca
  • Fonction : Auteur
Dominique Thiaudière
  • Fonction : Auteur
Frederic Lioté
  • Fonction : Auteur
Michel Daudon

Résumé

We aimed to examine the presence of Zn, a trace element, in osteoarthritis (OA) cartilage and meniscus from patients undergoing total knee joint replacement for primary OA. We mapped Ca2+ and Zn2+ at the mesoscopic scale by X-ray fluorescence microanalysis (μX-ray) to determine the spatial distribution of the 2 elements in cartilage, μX-ray absorption near edge structure spectroscopy to identify the Zn species, and μX-ray diffraction to determine the chemical nature of the calcification. Fourier transform infrared spectroscopy was used to determine the chemical composition of cartilage and meniscus. Ca2+ showed a heterogeneous spatial distribution corresponding to the calcifications within cartilage (or meniscus) or at their surface. At least 2 Zn2+ species were present: the first may correspond to Zn embedded in protein (different Zn metalloproteins are known to prevent calcification in biological tissues), and the second may be associated with a Zn trap in or at the surface of the calcification. Calcification present in OA cartilage may significantly modify the spatial distribution of Zn; part of the Zn may be trapped in the calcification and may alter the associated biological function of Zn metalloproteins.
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Dates et versions

hal-01468401 , version 1 (15-02-2017)

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Arnaud Dessombz, Christelle Nguyen, Hang-Korng Ea, Stephan Rouzière, Eddy Foy, et al.. Combining μ X-ray fluorescence, μXANES and μXRD to shed light on Zn2+ cations in cartilage and meniscus calcifications. Journal of Trace Elements in Medicine and Biology, 2013, 27 (4), pp.326-333. ⟨10.1016/j.jtemb.2013.02.001⟩. ⟨hal-01468401⟩
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