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Article Dans Une Revue Methods in Molecular Biology Année : 2015

Examination of galectin-induced lattice formation on early B-cell development.

Résumé

Galectin-1 (GAL1) is a pre-B cell receptor (pre-BCR) ligand that induces pre-BCR clustering and leads to efficient pre-B cell proliferation and differentiation in the bone marrow. To study pre-BCR-GAL1 interactions and its functional consequence on the early steps of the B cell development, we combine structural nuclear magnetic resonance (NMR) approaches and B cell biology techniques. NMR is applied to identify the residues involved in pre-BCR-GAL1 interactions by monitoring chemical shift perturbations when the complex is formed. This structural information is then used at the cellular level to target specifically the complex formation during GAL1-induced pre-BCR clustering and lattice formation, using immunofluorescence techniques. Moreover, an in vivo assay was set up to study the consequence of synapse formation on the early steps of B cell development.
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Dates et versions

hal-01458188 , version 1 (06-02-2017)

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Stephane J C Mancini, Latifa El Antak, Annie Boned, Marion Espeli, Francoise Guerlesquin, et al.. Examination of galectin-induced lattice formation on early B-cell development.. Methods in Molecular Biology, 2015, 1207, pp.169--84. ⟨10.1007/978-1-4939-1396-1_11⟩. ⟨hal-01458188⟩
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