Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism - Archive ouverte HAL
Article Dans Une Revue Nucleic Acids Research Année : 2007

Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism

Résumé

In contrast to Escherichia coli no molecular mechanism controlling the biosynthesis of ribosomal proteins has been elucidated in Gram-positive organisms. Here we show that the expression of the Bacillus subtilis infC-rpmI-rplT operon encoding translation factor IF3 and the ribosomal proteins L35 and L20 is autoregulated by a complex transcription attenuation mechanism. It implicates a 200-bp leader region upstream of infC which contains two conserved regulatory elements, one of which can act as a transcription terminator. Using in vitro and in vivo approaches we show that expression of the operon is regulated at the level of transcription elongation by a change in the structure of the leader mRNA which depends upon the presence of ribosomal protein L20. L20 binds to a phylogenetically conserved domain and provokes premature transcription termination at the leader terminator. Footprint and toeprint experiments support a regulatory model involving molecular mimicry between the L20-binding sites on 23S rRNA and the mRNA. Our data suggest that Nomura’s model of ribosomal protein biosynthesis based on autogenous control and molecular mimicry is also valid in Gram-positive organisms
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hal-01454015 , version 1 (29-05-2020)

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Nasslie Choonee, Sergine Even, Lena Zig, Harald Putzer. Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism. Nucleic Acids Research, 2007, 35 (5), pp.1578-1588. ⟨10.1093/nar/gkm011⟩. ⟨hal-01454015⟩
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