Article Dans Une Revue Open Biology Année : 2016

The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages

Anne-Laure Roux
  • Fonction : Auteur
Albertus Viljoen
Aicha Bah
  • Fonction : Auteur
Roxane Simeone
Audrey Bernut
Laura Laencina
  • Fonction : Auteur
Therese Deramaudt
  • Fonction : Auteur
Martin Rottman
Jean-Louis Gaillard
  • Fonction : Auteur
Laleh Majlessi
  • Fonction : Auteur
Roland Brosch
Fabienne Girard-Misguich
  • Fonction : Auteur
Isabelle Vergne
Laurent Kremer
Jean-Louis Herrmann

Résumé

Mycobacterium abscessus is a pathogenic, rapidly growing mycobacterium responsible for pulmonary and cutaneous infections in immunocompetent patients and in patients with Mendelian disorders, such as cystic fibrosis (CF). Mycobacterium abscessus is known to transition from a smooth (S) morphotype with cell surface-associated glycopeptidolipids (GPL) to a rough (R) morphotype lacking GPL. Herein, we show that M. abscessus S and R variants are able to grow inside macrophages and are present in morphologically distinct phagosomes. The S forms are found mostly as single bacteria within phagosomes characterized by a tightly apposed phagosomal membrane and the presence of an electron translucent zone (ETZ) surrounding the bacilli. By contrast, infection with the R form leads to phagosomes often containing more than two bacilli, surrounded by a loose phagosomal membrane and lacking the ETZ. In contrast to the R variant, the S variant is capable of restricting intraphagosomal acidification and induces less apoptosis and autophagy. Importantly, the membrane of phagosomes enclosing the S forms showed signs of alteration, such as breaks or partial degradation. Although not frequently encountered, these events suggest that the S form is capable of provoking phagosomecytosol communication. In conclusion, M. abscessus S exhibits traits inside macrophages that are reminiscent of slow-growing mycobacterial species.

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hal-01438481 , version 1 (02-07-2025)

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Anne-Laure Roux, Albertus Viljoen, Aicha Bah, Roxane Simeone, Audrey Bernut, et al.. The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages. Open Biology, 2016, 6 (11), ⟨10.1098/rsob.160185⟩. ⟨hal-01438481⟩
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