IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Oncology Année : 2016

IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression

Résumé

IGF-2 mRNA binding protein 3 (IGF2BP3, IMP-3) is a well-known post-transcriptional regulatory factor of gene expression, mainly involved in embryonic development and oncogenesis. We have previously demonstrated that a subset of IMP-3 targets, such as the mRNAs of cyclins D1, D3 and G1, are positively regulated by IMP-3, and that this regulation depends on nuclear localization of IMP-3. In the present study, we show that as a first step following a knock-down of IMP-3, the protein levels of the cyclins rapidly decrease, while their mRNAs remain stable and associated with the polyribosomes, though not translated. We have elucidated the molecular mechanisms of this regulation, demonstrating that IMP-3 and its protein partners ILF3/NF90 and PTBP1 bind to the 3'UTRs of the cyclin mRNAs and protect them from the translational repression induced by miRNA-dependent recruitment of AGO2/GW182 complex in human cancer cells.

Dates et versions

hal-01403341 , version 1 (25-11-2016)

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Citer

Evgeny Deforzh, Thaiz Vargas, Jeremie Kropp, Marie Vandamme, Guillaume Pinna, et al.. IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. International Journal of Oncology, 2016, ⟨10.3892/ijo.2016.3750⟩. ⟨hal-01403341⟩
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