Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation
Résumé
Background: The objective of the work was to characterize fungal endophytes from aerial parts of Vanilla planifolia. Also, to establish their biotransformation abilities of flavor-related metabolites. This was done in order to find a potential role of endophytes on vanilla flavors. Results: Twenty three MOTUs were obtained, representing 6 fungal classes. Fungi from green pods were cultured on mature green pod based media for 30 days followed by 1 H NMR and HPLC-DAD analysis. All fungi from pods consumed metabolized vanilla flavor phenolics. Though Fusarium proliferatum was recovered more often (37.6 % of the isolates), it is Pestalotiopsis microspora (3.0 %) that increased the absolute amounts (quantified by 1 H NMR in μmol/g DW green pods) of vanillin (37.0 × 10 −3), vanillyl alcohol (100.0 × 10 −3), vanillic acid (9.2 × 10 −3) and p-hydroxybenzoic acid (87.9 × 10 −3) by significant amounts.
Domaines
Biologie végétale| Origine | Fichiers éditeurs autorisés sur une archive ouverte |
|---|
Loading...