A bioenergetical analysis of manoeuvrability and agility performance for wheelchair players is inexistent. It was aimed at comparing the physiological responses and performance obtained from the octagon multistage field test (MFT) and the modified condition in " 8 form " (MFT-8). Sixteen trained wheelchair basketball players performed both tests in randomized condition. The levels performed (end-test score), peak values of oxygen uptake (VO 2peak), minute ventilation (V Epeak), heart rate (HR peak), peak and relative blood lactate (Δ[Lact − ] = peak – rest values), and the perceived rating exertion (RPE) were measured. MFT-8 induced higher VO 2peak and V Epeak values compared to MFT (VO 2peak : 2.5 ± 0.6 versus 2.3 ± 0.6 L⋅min −1 and V Epeak : 96.3 ± 29.1 versus 86.6 ± 23.4 L⋅min −1 ; í µí±ƒ < 0.05) with no difference in other parameters. Significant relations between V Epeak and end-test score were correlated for both field tests (í µí±ƒ < 0.05). At exhaustion, MFT attained incompletely VO 2peak and V Epeak. Among experienced wheelchair players, MFT-8 had no effect on test performance but generates higher physiological responses than MFT. It could be explained by demands of wheelchair skills occurring in 8 form during the modified condition.