Article Dans Une Revue Journal of Pharmacological and Toxicological Methods Année : 2016

Injection of insect membrane in Xenopus oocyte: An original method for the pharmacological characterization of neonicotinoid insecticides

Résumé

Insect nicotinic acetylcholine receptors (nAChRs) represent a major target of insecticides, belonging to the neonicotinoid family. However, the pharmacological profile of native nAChRs is poorly documented, mainly because of a lack of knowledge of their subunit stoichiometry, their tissue distribution and the weak access to nAChR-expressing cells. In addition, the expression of insect nAChRs in heterologous systems remains hard to achieve. Therefore, the structure–activity characterization of nAChR-targeting insecticides is made difficult. The objective of the present study was to characterize insect nAChRs by an electrophysiological approach in a heterologous system naturally devoid of these receptors to allow a molecular/cellular investigation of the mode of action of neonicotinoids.

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Dates et versions

hal-01388744 , version 1 (27-10-2016)

Identifiants

Citer

Lucille Crespin, Christian Legros, Olivier List, Hélène Tricoire-Leignel, César Mattei. Injection of insect membrane in Xenopus oocyte: An original method for the pharmacological characterization of neonicotinoid insecticides. Journal of Pharmacological and Toxicological Methods, 2016, 77, pp.10-16. ⟨10.1016/j.vascn.2015.09.004⟩. ⟨hal-01388744⟩
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