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Article Dans Une Revue Journal of Proteomics Année : 2012

Plasma membrane proteomes of differentially matured dendritic cells identified by LC–MS/MS combined with iTRAQ labelling

Résumé

Dendritic cells (DCs) play a pivotal role in polarising Th lymphocyte subsets but it is unclear whatmolecular events occur when DCs generate Th2-type responses. Here, we analysed plasma membrane-enriched fractions from immature, pro-Th1 and pro-Th2 DCs and used a combination of iTRAQ labelling and LC–MS/MS to quantify changes in the proteomes. Analysiswas performed on triplicate biological samples and changes verified by flow cytometry. MHC class II molecules and CD29 were up-regulated in pro-Th1 DCs whilst CD18 and CD44 were upregulated in pro-Th2 DCs. One of the most down-regulated molecules in pro-Th1 DCs was YM-1 whilst the greatest decrease in pro-Th2 DCs was NAP-22. Other molecules upregulated in pro-Th2 DC compared to pro-Th1 DCs included some potentially involved in protein folding during antigen processing (clathrin and Rab-7), whilst other non-membrane proteins such as enzymes/transporters related to cellmetabolism(malate dehydrogenase, pyruvate kinase, and ATPase Na+/K+) were also recorded. This suggests that pro-Th2 DCs are more metabolically activewhile pro-Th1 DCs have amature ‘end state’. Overall, althoughseveralmoleculeswere preferentially expressed on pro-Th2 DCs, our proteomics data support the view of a ‘limited maturation’ of pro-Th2 DCs compared to pro-Th1 DCs.

Domaines

Immunologie

Dates et versions

hal-01369365 , version 1 (20-09-2016)

Identifiants

Citer

Stéphanie Ferret-Bernard, William Castro-Borges, Adam A. Dowle, David E. Sanin, Peter C. Cook, et al.. Plasma membrane proteomes of differentially matured dendritic cells identified by LC–MS/MS combined with iTRAQ labelling. Journal of Proteomics, 2012, 75 (3), pp.938-948. ⟨10.1016/j.jprot.2011.10.010⟩. ⟨hal-01369365⟩
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