General Strategy for the Design of DNA Coding Sequences Applied to Nanoparticle Assembly - Archive ouverte HAL
Article Dans Une Revue Langmuir Année : 2016

General Strategy for the Design of DNA Coding Sequences Applied to Nanoparticle Assembly

Résumé

The DNA-directed assembly of nano-objects has been the subject of many recent studies as a means to construct advanced nanomaterial architectures. Although much experimental in silico work has been presented and discussed, there has been no in-depth consideration of the proper design of single-strand sticky termination of DNA sequences, noted as ssST, which is important in avoiding self-folding within one DNA strand, unwanted strand-to-strand interaction, and mismatching. In this work, a new comprehensive and computationally efficient optimization algorithm is presented for the construction of all possible DNA sequences that specifically prevents these issues. This optimization procedure is also effective when a spacer section is used, typically repeated sequences of thymine or adenine placed between the ssST and the nano-object, to address the most conventional experimental protocols. We systematically discuss the fundamental statistics of DNA sequences considering complementarities limited to two (or three) adjacent pairs to avoid self-folding and hybridization of identical strands due to unwanted complements and mismatching. The optimized DNA sequences can reach maximum lengths of 9 to 34 bases depending on the level of applied constraints. The thermodynamic properties of the allowed sequences are used to develop a ranking for each design. For instance, we show that the maximum melting temperature saturates with 14 bases under typical solvation and concentration conditions. Thus, DNA ssST with optimized sequences are developed for segments ranging from 4 to 40 bases, providing a very useful guide for all technological protocols. An experimental test is presented and discussed using the aggregation of Al and CuO nanoparticles and is shown to validate and illustrate the importance of the proposed DNA coding sequence optimization. ■ INTRODUCTION The interest in DNA nanotechnology to program the assembly of nanoparticles into macroscopic nanocomposites emerged in the 1990s. 1,2 Undoubtedly, the controlled interplay of DNA complementary and noncomplementary strands made DNA nanotechnologies one of the most powerful bottom-up approaches to building hierarchical architectures of nano-objects (noble metals, semiconductors, oxides, and polymers) leading to an almost infinite variety of high-performance programmable DNA/nanoparticles hybrid materials. One mainstream DNA-based assembly approach consists of directing the assembly of colloids of interest, mostly gold nanoparticles, by taking advantage of the thiol/metal chemistry 3 to covalently attach DNA strands to nanoparticle surfaces. Other chemical alternatives have also been investigated , such as antigen/antibody-like binding. 4,5 Since the seminal work by Alavisatos and Mirkin 1,2 on gold nanoparticles, many DNA/nanoparticle assembly processes have been reported, notably by varying the DNA length and processing conditions and with a consideration of other materials for applications in catalytics, 6 spectroscopy, 7−10 optical devices,
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hal-01368340 , version 1 (19-06-2019)

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Théo Calais, Vincent Baijot, Mehdi Djafari Rouhani, David Gauchard, Yves J Chabal, et al.. General Strategy for the Design of DNA Coding Sequences Applied to Nanoparticle Assembly. Langmuir, 2016, 32 (37), pp.9676-9686. ⟨10.1021/acs.langmuir.6b02843⟩. ⟨hal-01368340⟩
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