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Article Dans Une Revue Journal of Physical Chemistry Letters Année : 2015

Hyperpolarized Water to Study Protein-Ligand Interactions

Résumé

The affinity between a chosen target protein and small molecules is a key aspect of drug discovery. Screening by popular NMR methods such as Water-LOGSY suffers from low sensitivity and from false positives caused by aggregated or denatured proteins. This work demonstrates that the sensitivity of Water-LOGSY can be greatly boosted by injecting hyperpolarized water into solutions of proteins and ligands. Ligand binding can be detected in a few seconds, whereas about 30 min is usually required without hyperpolarization. Hyperpolarized water also enhances proton signals of proteins at concentrations below 20 M so that one can verify in a few seconds whether the proteins remain intact or have been denatured

Dates et versions

hal-01366198 , version 1 (14-09-2016)

Identifiants

Citer

Quentin Chappuis, Jonas Milani, Basile Vuichoud, Aurélien Bornet, Alvar D. Gossert, et al.. Hyperpolarized Water to Study Protein-Ligand Interactions. Journal of Physical Chemistry Letters, 2015, 6 (9), pp.1674-1678. ⟨10.1021/acs.jpclett.5b00403⟩. ⟨hal-01366198⟩
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