PBPK model of methotrexate in cerebrospinal fluid ventricles using a combined microdialysis and MRI acquisition - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue European Journal of Pharmaceutics and Biopharmaceutics Année : 2016

PBPK model of methotrexate in cerebrospinal fluid ventricles using a combined microdialysis and MRI acquisition

Résumé

The objective of the study was to evaluate the distribution of methotrexate (MTX) in cerebrospinal fluid (CSF) lateral ventricles and in cisterna magna after 3rd intraventricular CSF administration in a rabbit model.MTX or gadolinium chelate (Gd-DOTA) was administered in the 3rd ventricle with a local microdialysis to study the pharmacokinetics at the site of administration and with a simultaneous magnetic resonance imaging (MRI) acquisition in the 3rd ventricle, the lateral ventricles and in the cisterna magna. A specific CSF Physiologically Based Pharmacokinetic (PBPK) model was then extrapolated for MTX from Gd-DOTA data.The relative contribution of elimination and distribution processes to the overall disposition of MTX and Gd-DOTA in the 3rd ventricle was similar (i.e., around 60% for CLE and 40% for CLI) suggesting that Gd-DOTA was a suitable surrogate marker for MTX disposition in ventricular CSF. The PBPK predictions for MTX both in CSF of the 3rd ventricle and in plasma were in accordance with the in vivo results.The present study showed that the combination of local CSF microdialysis with MRI acquisition of the brain ventricles and a PBPK model could be a useful methodology to estimate the drug diffusion within CSF ventricles after direct brain CSF administration. Such a methodology would be of interest to clinicians for a rationale determination and optimization of drug dosing parameters in the treatment of leptomeningeal metastases.
Fichier principal
Vignette du fichier
PBPK model of methotrexate in cerebrospinal fluid.pdf (2.17 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01335562 , version 1 (11-12-2016)

Identifiants

Citer

Nolwenn Brandhonneur, Fanny Noury, Arnaud Bruyère, Hervé Saint-Jalmes, Pascal Le Corre. PBPK model of methotrexate in cerebrospinal fluid ventricles using a combined microdialysis and MRI acquisition. European Journal of Pharmaceutics and Biopharmaceutics, 2016, 104, pp.117-130. ⟨10.1016/j.ejpb.2016.04.012⟩. ⟨hal-01335562⟩
355 Consultations
432 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More