Towards the in-vivo automated assessment of nasal cilia mobility
Élodie Puybareau
(1)
,
Emilie Bequignon
(2)
,
Mathieu Bottier
(2)
,
Gabriel Pelle
(2)
,
Bruno Louis
(2)
,
Estelle Escudier
(3)
,
Jean-François Papon
(2)
,
Laurent Najman
(1)
,
Hugues Talbot
(1)
,
André Coste
(2)
Élodie Puybareau
- Fonction : Auteur
- PersonId : 4067
- IdHAL : elodie-puybareau
- ORCID : 0000-0002-2748-6624
- IdRef : 224942298
Bruno Louis
- Fonction : Auteur
- PersonId : 743307
- IdHAL : bruno-louis
- ORCID : 0000-0001-9587-9932
- IdRef : 110436296
Estelle Escudier
- Fonction : Auteur
- PersonId : 1213534
- IdHAL : estelle-escudier
- ORCID : 0000-0002-1569-8072
- IdRef : 033701997
Laurent Najman
- Fonction : Auteur
- PersonId : 28
- IdHAL : laurent-najman
- ORCID : 0000-0002-6190-0235
- IdRef : 087172712
Hugues Talbot
- Fonction : Auteur
- PersonId : 1939
- IdHAL : hugues-talbot
- ORCID : 0000-0002-2179-3498
- IdRef : 181843056
Résumé
Introduction. Cilia motility is an important diagnostic feature for various nasal diseases, both of acquired and genetic origin. So far this assessment has been performed ex-vivo in nasal samples. Sampling is invasive and may damage cilia, and ex-vivo measurements may not always reflect the in-vivo cilia function. In this work we investigated the possibility of assessing cilia motility in vivo in humans with a preliminary study using confocal micro-endoscopy.
Materials and methods. We used the high-power laser confocal endoscope developed by Mauna Kea Technology (MKT). This device has a 1µm spatial resolution and a temporal resolution varying between 8 and 90 Hz. Ex-vivo pig trachea samples and human nasal biopsy samples were labelled with fluorescent marker Octadecyl Rhodamine B Chloride (R18). Beating cilia were easily identified allowing the acquisition of 40 videos ready for analysis. Using in-house software that estimates frequencies based on luminance variation in a small window and FFT analysis, we evaluated cilia and compared the beat frequency with ground truth.
Results. We validated our estimations on all sequences acquired between 30 and 90Hz. Videos acquired at less than 30Hz did not offer sufficient temporal resolution We only observed occasional errors when the software identified a harmonic oscillation instead of the fundamental frequency.
Domaines
Imagerie médicaleFormat du dépôt | Annexe |
---|---|
Type de dépôt | Communication dans un congrès |
Titre |
en
Towards the in-vivo automated assessment of nasal cilia mobility
|
Résumé |
en
Introduction. Cilia motility is an important diagnostic feature for various nasal diseases, both of acquired and genetic origin. So far this assessment has been performed ex-vivo in nasal samples. Sampling is invasive and may damage cilia, and ex-vivo measurements may not always reflect the in-vivo cilia function. In this work we investigated the possibility of assessing cilia motility in vivo in humans with a preliminary study using confocal micro-endoscopy.
Materials and methods. We used the high-power laser confocal endoscope developed by Mauna Kea Technology (MKT). This device has a 1µm spatial resolution and a temporal resolution varying between 8 and 90 Hz. Ex-vivo pig trachea samples and human nasal biopsy samples were labelled with fluorescent marker Octadecyl Rhodamine B Chloride (R18). Beating cilia were easily identified allowing the acquisition of 40 videos ready for analysis. Using in-house software that estimates frequencies based on luminance variation in a small window and FFT analysis, we evaluated cilia and compared the beat frequency with ground truth.
Results. We validated our estimations on all sequences acquired between 30 and 90Hz. Videos acquired at less than 30Hz did not offer sufficient temporal resolution We only observed occasional errors when the software identified a harmonic oscillation instead of the fundamental frequency.
|
Auteur(s) |
Élodie Puybareau
1
, Emilie Bequignon
2
, Mathieu Bottier
2
, Gabriel Pelle
2
, Bruno Louis
2
, Estelle Escudier
3
, Jean-François Papon
2
, Laurent Najman
1
, Hugues Talbot
1
, André Coste
2
1
LIGM -
Laboratoire d'Informatique Gaspard-Monge
( 3210 )
- Université de Paris-Est Marne-la-Vallée, Cité Descartes, Bâtiment Copernic, 5 bd Descartes, 77454 Marne-la-Vallée Cedex 2
- France
2
INSERM U955, équipe 13
( 390139 )
-
- France
3
CHU Trousseau [APHP]
( 360410 )
- 26 Avenue du Dr Arnold Netter, 75012 Paris
- France
|
Langue du document |
Anglais
|
Vulgarisation |
Non
|
Actes |
Non
|
Comité de lecture |
Oui
|
Invité |
Non
|
Audience |
Internationale
|
Titre du congrès |
Congress of the European Rhinologic Society
|
Date début congrès |
2016-07-03
|
Date fin congrès |
2016-07-07
|
Ville |
Stockholm
|
Pays |
Suède
|
Domaine(s) |
|
Origine :
Fichiers produits par l'(les) auteur(s)
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