Hyperspectral coherent Raman imaging – principle, theory, instrumentation, and applications to life sciences - Archive ouverte HAL
Article Dans Une Revue Journal of Raman Spectroscopy Année : 2016

Hyperspectral coherent Raman imaging – principle, theory, instrumentation, and applications to life sciences

Résumé

Coherent Raman scattering microscopy such as coherent anti-Stokes Raman scattering and stimulated Raman scattering microscopy boosts the weak Raman signal and enables us to perform label-free visualization of the molecular distribution and its dynamical behavior in living cells and tissues with high speed. In comparison with fluorescence imaging, cells and tissues can be visualized without specifying the target molecule. In this review, we describe the characteristics of a hyperspectral coherent Raman imaging method, which is capable of acquiring both spectra and images simultaneously, and review applications to life sciences. Copyright © 2015 John Wiley & Sons, Ltd.

Dates et versions

hal-01321780 , version 1 (26-05-2016)

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Citer

Hideaki Kano, Hiroki Segawa, Masanari Okuno, Philippe Leproux, Vincent Couderc. Hyperspectral coherent Raman imaging – principle, theory, instrumentation, and applications to life sciences. Journal of Raman Spectroscopy, 2016, 47 (1), pp.116-123. ⟨10.1002/jrs.4853⟩. ⟨hal-01321780⟩
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