Article Dans Une Revue World Journal of Microbiology and Biotechnology Année : 2014

Production of the Phanerochaete flavido-alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation

Résumé

We investigated the expression of Phanerochaete flavido-alba laccase gene in Aspergillus niger and the physical and biochemical properties of the recombinant enzyme (rLac-LPFA) in order to test it for synthetic dye biotransformation. A. niger was able to produce high levels of active recombinant enzyme (30 mgL(-1)), whose identity was further confirmed by immunodetection using Western blot analysis and N-terminal sequencing. Interestingly, rLac-LPFA exhibited an improved stability at pH (2-9) and organic solvents tested. Furthermore, the percentage of decoloration and biotransformation of synthetic textile dyes, Remazol Brilliant Blue R (RBBR) and Acid Red 299 (NY1), was higher than for the native enzyme. Its high production, simple purification, high activity, stability and ability to transform textile dyes make rLac-LPFA a good candidate for industrial applications.

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Dates et versions

hal-01268541 , version 1 (04-02-2016)

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Lamiae Benghazi, Eric Record, Antonio Suarez, Jose A. Gomez-Vidal, Jose Martinez, et al.. Production of the Phanerochaete flavido-alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation. World Journal of Microbiology and Biotechnology, 2014, 30 (1), pp.201-211. ⟨10.1007/s11274-013-1440-z⟩. ⟨hal-01268541⟩
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