Introduction: Early nutrition through maternal diet during pregnancy and breastfeeding was shown to have an impact on obesity in childhood. Experiments on animal models have also indicated alterations regarding taste preferences. In addition, salivary physiology has a documented role in taste preferences and food intake in rodents. The objective of this study was therefore to study the impact of maternal diet on salivary glands’ protein profiles of the offspring. Materials and methods: Female rats received during gestation and lactation either a control (C) or a high fat / high sugar diet (WD for “Western Diet”). 18 offspring rats (9C/9WD) were sacrificed at 25 days of age, i.e. shortly after weaning. Protein expression in their submandibular glands was studied by 2D electrophoresis, and proteins differentially expressed (p<0.05) between the two groups were identified by LC-MS/MS. Results: Out of 413 spots detected and semi-quantified, 16 spots were differentially expressed between WD and C young rats. The submandibular glands of WD animals were characterized in particular by a higher abundance of proteins with chaperone functions (Hsc70, HSP60, protein disulfide isomerase), annexin A6, proteasome, or enzymes previously described as responding to nutrient intake (2-oxoisovalerate dehydrogenase, adenosylhomocysteinase). Discussion/conclusion: We here demonstrate the plasticity of offspring salivary glands in response to maternal diet. The link with saliva secretion and ingestive behaviour (sweet and fat preference) deserves further investigation.