RNA synthetic biology inspired from bacteria: construction of transcription attenuators under antisense regulation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Physical Biology Année : 2009

RNA synthetic biology inspired from bacteria: construction of transcription attenuators under antisense regulation

Hervé Isambert

Résumé

Among all biopolymers, ribonucleic acids or RNA have unique functional versatility, which led to the early suggestion that RNA alone (or a closely related biopolymer) might have once sustained a primitive form of life based on a single type of biopolymer. This has been supported by the demonstration of processive RNA-based replication and the discovery of 'riboswitches' or RNA switches, which directly sense their metabolic environment. In this paper, we further explore the plausibility of this 'RNA world' scenario and show, through synthetic molecular design guided by advanced RNA simulations, that RNA can also perform elementary regulation tasks on its own. We demonstrate that RNA synthetic regulatory modules directly inspired from bacterial transcription attenuators can efficiently activate or repress the expression of other RNA by merely controlling their folding paths 'on the fly' during transcription through simple RNA-RNA antisense interaction. Factors, such as NTP concentration and RNA synthesis rate, affecting the efficiency of this kinetic regulation mechanism are also studied and discussed in the light of evolutionary constraints. Overall, this suggests that direct coupling among synthesis, folding and regulation of RNAs may have enabled the early emergence of autonomous RNA-based regulation networks in absence of both DNA and protein partners.
Fichier non déposé

Dates et versions

hal-01239353 , version 1 (07-12-2015)

Identifiants

Citer

Alexandre Dawid, Bastien Cayrol, Hervé Isambert. RNA synthetic biology inspired from bacteria: construction of transcription attenuators under antisense regulation. Physical Biology, 2009, 6 (2), pp.025007. ⟨10.1088/1478-3975/6/2/025007⟩. ⟨hal-01239353⟩
71 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More