Exhaust emissions and fuel consumption of Heavy Duty Vehicles (HDVs) in urban and port areas were evaluated through a dedicated investigation. The HDV fleet composition and traffic driving from highways to the maritime port of Genoa and crossing the city were analysed. Typical urban trips linking highway exits to port gates and HDV mission profiles within the port area were defined. A validation was performed through on-board instru- mentation to record HDV instantaneous speeds in urban and port zones. A statistical pro- cedure enabled the building-up of representative speed patterns. High contrasts and specific driving conditions were observed in the port area. Representative speed profiles were then used to simulate fuel consumption and emissions for HDVs, using the Passenger car and Heavy duty Emission Model (PHEM). Complementary estimations were derived from Copert and HBEFA methodologies, allowing the comparison of different calculation approaches and scales. Finally, PHEM was implemented to assess the performances of EGR or SCR systems for NOX reduction in urban driving and at very low speeds. The method and results of the investigation are presented. Fuel consumption and pollu- tant emission estimation through different methodologies are discussed, as well as the necessity of characterizing very local driving conditions for appropriate assessment.