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Communication Dans Un Congrès Année : 2012

Use of the nuclear region ITS2 for arthropods species identification

Résumé

During the last decade, many barcode projects were launched for species identification by DNA sequencing in order to improve taxonomic knowledge of biodiversity and to try to assign sample individuals to species. In the case of Arthropods the most often used region is a part of the mitochondrial gene Cytochrome Oxydase I, which is often insufficient to reliably delineate and name the species. To confirm the identification of specimens it is necessary to use another DNA sequence. This sequence is very often the nuclear ribosomal internal transcribed spacer 2 (ITS2). The data presented here show the preliminary results of ITS2 sequencing on 40 arthropods species belonging todifferent orders (Coleoptera, Lepidoptera, Hymenoptera).The results presented show the difficulty of defining universal primers for this target ITS2 and the large variability of obtained sequences (presence of deletion, repeats, etc.). An example of sequences obtained for the genus Dendroctonus (Coleoptera, Curculionidae) is also supplied.
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Dates et versions

hal-01231303 , version 1 (06-06-2020)

Identifiants

  • HAL Id : hal-01231303 , version 1
  • PRODINRA : 183709

Citer

Béatrice Courtial, Marie-Anne Auger-Rozenberg, Alain Roques. Use of the nuclear region ITS2 for arthropods species identification. QBOL-EPPO Conference on DNA barcoding and diagnostic methods for plant pests, European and Mediterranean Plant Protection Organization - Organisation Européenne et Méditerranéenne pour la Protection des Plantes (EPPO). Paris, FRA., May 2012, Haarlem, Netherlands. 1 p. ⟨hal-01231303⟩

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