Theoretical and practical interest in UHPLC technology for 2D-LC
Abstract
Comprehensive two-dimensional liquid chromatography (LC x LC) has significantly improved the separation power of LC for complex samples. Our review opens with theoretical considerations about online LC x LC in order to explain why ultra-high-pressure LC (UHPLC) and more specifically, hightemperature (HT)-UHPLC are very convenient in the second dimension. We present some recent applications involving UHPLC in LC x LC. They cover a wide range of analytical fields, such as food analysis, life sciences, bioenergy and polymers
Keywords
2-DIMENSIONAL LIQUID-CHROMATOGRAPHY
ULTRA-HIGH PRESSURE
PEAK-CAPACITY
GRADIENT ELUTION
HYDROPHILIC INTERACTION
SYSTEMATIC OPTIMIZATION
PROGRAMMED ELUTION
BUFFERED ELUENTS
SEPARATION POWER
OFF-LINE
2D-LC
LC x LC
Comprehensive two-dimensional liquid chromatography (LC x LC)
Food analysis
High-temperature-UHPLC (HT-UHPLC)
HT-UHPLC
Polymer analysis
Two-dimensional liquid chromatography
(2D-LC)
UHPLC
Ultra-high-pressure liquid chromatography (UHPLC)