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Article Dans Une Revue Theriogenology Année : 2005

Role of seminal plasma in damage to turkey spermatozoa during in vitro storage

Résumé

In vitro storage of turkey spermatozoa is performed without consideration of the potential role of seminal plasma on sperm functions. We report the effects of seminal plasma on membrane permeability, lipid metabolism, energy status, motility and fertility of turkey spermatozoa stored at 4 or 20 °C. Phospholipid content (1077 nmol/109 spz versus 1219 nmol/109 spz at 48 h) and membrane permeability of spermatozoa were significantly damaged by the presence of seminal plasma after 48 h of storage at 4 °C, whereas damage to ATP content and fertility occurred earlier damaged by this presence (fertility after 24 h storage 51% with seminal plasma versus 71% without). At 20 °C, seminal plasma decreased the phospholipid content of spermatozoa in the first hour of storage (1326 nmol/109 spz versus 1636 nmol/109 spz). Twenty-four hours later, this effect was masked by intense lipid peroxidation. These results show that seminal plasma is deleterious to storage of turkey spermatozoa at 4 °C and is involved in phospholipid metabolism of spermatozoa. Lipid peroxidation could be responsible for the acceleration of the degradation of sperm phospholipids during storage at 20 °C. However, lipid peroxidation seems not to be active at 4 °C. In this case, we suggest that phospholipase activation may contribute to sperm degradation, especially in the presence of seminal plasma.

Dates et versions

hal-01225263 , version 1 (05-11-2015)

Identifiants

Citer

Véronique Douard, Dominique Hermier, Catherine Labbé, Michèle Magistrini, Elisabeth Blesbois. Role of seminal plasma in damage to turkey spermatozoa during in vitro storage. Theriogenology, 2005, 63 (1), pp.126-137. ⟨10.1016/j.theriogenology.2004.03.020⟩. ⟨hal-01225263⟩
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