Lack of dietary polyunsaturated fatty acids causes synapse dysfunction in the drosophila visual system - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2015

Lack of dietary polyunsaturated fatty acids causes synapse dysfunction in the drosophila visual system

Résumé

Polyunsaturated fatty acids (PUFAs) are essential nutrients for animals and necessary for the normal functioning of the nervous system. A lack of PUFAs can result from the consumption of a deficient diet or genetic factors, which impact PUFA uptake and metabolism. Both can cause synaptic dysfunction, which is associated with numerous disorders. However, there is a knowledge gap linking these neuronal dysfunctions and their underlying molecular mechanisms. Because of its genetic manipulability and its easy, fast, and cheap breeding, Drosophila melanogaster has emerged as an excellent model organism for genetic screens, helping to identify the genetic bases of such events. As a first step towards the understanding of PUFA implications in Drosophila synaptic physiology we designed a breeding medium containing only very low amounts of PUFAs. We then used the fly’s visual system, a well-established model for studying signal transmission and neurological disorders, to measure the effects of a PUFA deficiency on synaptic function. Using both visual performance and eye electrophysiology, we found that PUFA deficiency strongly affected synaptic transmission in the fly’s visual system. These defects were rescued by diets containing omega-3 or omega-6 PUFAs alone or in combination. In summary, manipulating PUFA contents in the fly’s diet was powerful to investigate the role of these nutrients on the fly´s visual synaptic function. This study aims at showing how the first visual synapse of Drosophila can serve as a simple model to study the effects of PUFAs on synapse function. A similar approach could be further used to screen for genetic factors underlying the molecular mechanisms of synaptic dysfunctions associated with altered PUFA levels.
Fichier principal
Vignette du fichier
ziegler_2015_vol10_e0135353_plosone_{862666B0-4000-4384-922E-1CF77E88E752}.pdf (1.72 Mo) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...

Dates et versions

hal-01222420 , version 1 (29-10-2015)

Licence

Paternité

Identifiants

Citer

Anna Ziegler, Cindy Ménagé, Stéphane Grégoire, Thibault Garcia, Jean-François Ferveur, et al.. Lack of dietary polyunsaturated fatty acids causes synapse dysfunction in the drosophila visual system. PLoS ONE, 2015, 10 (8), pp.1-15. ⟨10.1371/journal.pone.0135353⟩. ⟨hal-01222420⟩
60 Consultations
111 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More