Design of Artificial Metabolisms in Layered Nanomaterials for the Enzymatic Synthesis of Phosphorylated Sugars. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue ChemCatChem Année : 2015

Design of Artificial Metabolisms in Layered Nanomaterials for the Enzymatic Synthesis of Phosphorylated Sugars.

Résumé

Biohybrid nanoreactors operating in one-pot cascade reactions were designed by co-immobilization of enzymes in an inorganic layered matrix, namely, layered double hydroxides. These biohybrid systems were devoted to prepare dihydroxyacetone phosphate (DHAP) and phosphorylated sugars through stereoselective C−C bond formation. In the first system, two kinases were exploited for the in situ generation of DHAP. Increasing the complexity, the second nano-bioreactor combined up to four enzymes to lead to d-fructose-6-phosphate from the aldol-catalyzed addition of dihydroxyacetone to d-glyceraldehyde-3-phosphate generated in situ from DHAP. The biohybrid catalyst showed the same reaction rate as that of the free enzymes and was reusable.

Domaines

Chimie

Dates et versions

hal-01213630 , version 1 (08-10-2015)

Identifiants

Citer

Rima Mahdi, Christine Guérard-Hélaine, Vanessa Prévot, Véronique de Berardinis, Claude Forano, et al.. Design of Artificial Metabolisms in Layered Nanomaterials for the Enzymatic Synthesis of Phosphorylated Sugars.. ChemCatChem, 2015, 7, pp.3110-3115. ⟨10.1002/cctc.201500606⟩. ⟨hal-01213630⟩
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