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Chapitre D'ouvrage Methods in Molecular Biology Année : 2015

Pulsed Field Gel Electrophoresis for Dairy [i]Propionibacteria[/i]

Résumé

Pulsed fi eld gel electrophoresis (PFGE) is a technique using alternating electric fi elds to migrate high molecular weight DNA fragments with a high resolution. This method consists of the digestion of bacterial chromosomal DNA with rare-cutting restriction enzymes and in applying an alternating electrical current between spatially distinct pairs of electrodes. DNA molecules migrate at different speeds according to the size of the fragments. Among other things, this technique is considered as the “gold standard” for genotyping, genetic fi ngerprinting, epidemiological studies, genome size estimation, and studying radiationinduced DNA damage and repair. This chapter describes a PFGE method that can be used to differentiate dairy propionibacteria
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Dates et versions

hal-01209809 , version 1 (02-10-2015)

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Citer

Victoria Chuat, Rosangela de Freitas, Marion Dalmasso. Pulsed Field Gel Electrophoresis for Dairy [i]Propionibacteria[/i]. Pulse Field Gel Electrophoresis, 1301 chapitre 21, Springer Protocol, Humana Press, 278 p., 2015, Methods in Molecular Biology, 978-1-4939-2599-5. ⟨10.1007/978-1-4939-2599-5_21⟩. ⟨hal-01209809⟩
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