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Poster De Conférence Année : 2013

Digestion in vitro dynamique du lait maternel : effet de la pasteurisation

Résumé

Human milk is the ideal food for infant nutrition and optimal growth. This is especially true for preterm neonates that are preferentially fed donor milk when their own mother milk is not available. For sanitary reasons, donor milk is pasteurized. This may have structural and biochemical consequences on the maternal milk emulsion and affect digestive kinetics. This has been rarely investigated. Thus that was the aim of our study. Mature human milk was collected and stored at -20°C for 2.5 months. Half of it was then digested as is (fresh milk). The other half was pasteurized (62.5°C, 30 min) and frozen for another 10 days prior digestion. An in vitro dynamic system was used to simulate the gastric and intestinal digestion phases of a 1-month old newborn. Structural changes of digested samples were evaluated by confocal microscopy and laser light scattering. Proteolysis and lipolysis were monitored over 180 min. Hydrolysis kinetics were followed by SDS-PAGE and Thin-Layer Chromatography methods, followed by densitometry for semi-quantitative estimations. Pasteurization impacted the emulsion structure by increasing the protein susceptibility to acid aggregation especially on the milk fat globule membrane. In the gastric compartment, we observed a faster hydrolysis of caseins (κ, β) and lactoferrin in pasteurized milk than in fresh milk. In the intestinal phase, most proteins were digested during the first 30 min. On the contrary, gastric digestion of lipids was slower in pasteurized milk than in fresh milk. This was probably due to the protein aggregation at the interface of the fat globule membrane, acting as a protective barrier and to an inactivation of the endogenous human milk lipase by pasteurization. These results demonstrate that pasteurization impacts the rate of proteolysis and lipolysis. The physiologic and metabolic impacts remain unknown, especially in newborns exclusively fed pasteurized milk.
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Dates et versions

hal-01209452 , version 1 (02-10-2015)

Identifiants

  • HAL Id : hal-01209452 , version 1
  • PRODINRA : 194134

Citer

Olivia Ménard, Amélie Deglaire, Claire Bourlieu-Lacanal, Sophie Chever, Alain Beuchée, et al.. Digestion in vitro dynamique du lait maternel : effet de la pasteurisation. Journées nationales de l'association des lactariums de France, Jun 2013, Rennes, France. , 2013. ⟨hal-01209452⟩
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