Straightforward Synthesis of Terminally Phosphorylated L-Sugars via Multienzymatic Cascade Reactions.
Résumé
Two kinases and the key fructose-6-phosphate aldolase (FSA) were involved in a one-pot multienzymatic procedure to obtain rare L-sugars. Starting from simple achiral substrates such as glycolaldehyde and formaldehyde, L-glyceraldehyde-3-phosphate (L-G3P) was generated. When this latter compound was used as an FSA acceptor substrate along with four different donor compounds, four terminally phosphorylated L-monosaccharides from C5 to C7 chain lengths were prepared in good isolated yields.