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Article Dans Une Revue Chemistry - A European Journal Année : 2015

Fluorescent Silica Nanoparticles with Multivalent Inhibitory Effects towards Carbonic Anhydrases

Résumé

Multifunctional silica nanoparticles decorated with fluorescent and sulfonamide carbonic anhydrase (CA) inhibitors were prepared and investigated as multivalent enzyme inhibitors against the cytosolic isoforms hCA I and II and the transmembrane tumor-associated ones hCA IX and XII. Excellent inhibitory effects were observed with these nanoparticles, with KI values in the low nanomolar range (6.2–0.67 nm) against all tested isozymes. A significant multivalency effect was seen for the inhibition of the monomeric enzymes hCA I and II compared to the dimeric hCA IX and hCA XII isoforms, where no multivalent effect was observed, suggesting that the multivalent binding is occurring through enzyme clustering.

Domaines

Matériaux
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Dates et versions

hal-01206436 , version 1 (28-01-2022)

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Nadia Touisni, Nasreddine Kanfar, Sebastien Ulrich,, Pascal Dumy, Claudiu T Supuran, et al.. Fluorescent Silica Nanoparticles with Multivalent Inhibitory Effects towards Carbonic Anhydrases. Chemistry - A European Journal, 2015, ⟨10.1002/chem.201501037⟩. ⟨hal-01206436⟩
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