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Article Dans Une Revue Molecular Microbiology Année : 2014

Phosphorylation of [i]Bacillus subtilis[/i] gene regulator AbrB modulates its DNA-binding properties

Résumé

AbrB is a global gene regulator involved in transition phase phenomena in Bacillus subtilis. It participates in a complex regulatory network governing the expression of stationary-phase functions. AbrB was previously found to be phosphorylated on serine 86 located close to its C-terminal oligomerization domain. Here we report that AbrB can be phosphorylated by three B. subtilis serine/threonine kinases expressed during the transition and stationary phase: PrkC, PrkD and YabT. Our in vitro findings suggest that AbrB phosphorylation impedes its DNA binding and abolishes binding cooperativity. In vivo we established that a phospho-mimetic mutation abrB S86D leads to a significant loss of AbrB control over several key target functions: exoprotease production, competence development and sporulation. A wider transcriptome analysis of abrBS86D and S86A mutant strains revealed deregulation of a large number of target genes. We therefore propose that AbrB phosphorylation serves as an additional input for fine-tuning the activity of this ambiactive gene regulator.

Dates et versions

hal-01204387 , version 1 (23-09-2015)

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Ahasanul Kobir, Sandrine Poncet-Mouturat, Vladimir Bidnenko, Olivier Delumeau, Carsten Jers, et al.. Phosphorylation of [i]Bacillus subtilis[/i] gene regulator AbrB modulates its DNA-binding properties. Molecular Microbiology, 2014, 92 (5), pp.1129-1141. ⟨10.1111/mmi.12617⟩. ⟨hal-01204387⟩
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