Protein complexes characterization in Arabidopsis thaliana by tandem affinity purification coupled to mass spectrometry analysis - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2014

Protein complexes characterization in Arabidopsis thaliana by tandem affinity purification coupled to mass spectrometry analysis

Résumé

Proteins are major elements participating in all the key functions of the cells. They rarely fulfill their physiological roles in an autonomous way but rather act as part of more complex cellular machines. Indeed they can bind different types of molecules (proteins, nucleic acids, metabolites, etc.), via stable or transient interactions, depending on their nature and functions. The identification of the molecular partners of a given protein is hence essential to better understand its roles, regulation, and mechanisms of action.This chapter describes the use of a tandem affinity purification approach followed by mass spectrometry analysis to try to identify and characterize the proteins involved in protein complexes in Arabidopsis thaliana and decipher some mechanisms of regulation of the modules. Important elements to consider in such an approach are first extensively exposed in the introduction. This technique, in combination with complementary approaches like yeast two-hybrid and bimolecular fluorescence complementation, can be an interesting source of data to identify and characterize in vivo protein complexes.
Fichier non déposé

Dates et versions

hal-01203960 , version 1 (23-09-2015)

Identifiants

Citer

Jean Bigeard, Delphine Pflieger, Jean Colcombet, Loic Gerard, Hakim Mireau, et al.. Protein complexes characterization in Arabidopsis thaliana by tandem affinity purification coupled to mass spectrometry analysis. Plant Map kinases, 1171, Springer Science + Business Media B.V., 2014, Methods in Molecular Biology, ⟨10.1007/978-1-4939-0922-3_18⟩. ⟨hal-01203960⟩
65 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More