Impacts of the origin and the treatment of organic waste recycled in agriculture on the fate and the availability of antibiotics in cultivated soils - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2014

Impacts of the origin and the treatment of organic waste recycled in agriculture on the fate and the availability of antibiotics in cultivated soils

Résumé

Veterinary and human antibiotics are found in manures and biosolids used in agriculture as organic amendments. The repeated introduction in cultivated soils of small amounts of antibiotics and by- products with organic waste may have an impact on microbial functions and communities, in particular by contributing to the acquisition of resistance against antibiotics. The effects of antibiotics on microorganisms are in theory linked to the bioavailable fraction of compounds in soil, which could be influenced by the treatment of organic waste (e.g. composting). The objective of this study was to follow the evolution of the (bio)available fraction of two antibiotics (sulfamethoxazole, SMX; ciprofloxacin, CIP) in soil after the application of two types of organic waste (compost of sewage sludge with green waste, bovine manure) currently used on the long-term field experiment QualiAgro (INRA–Veolia partnership 1998–2013). Soft chemical extractants were selected for this purpose and compared. Mixtures of soil (5 g dry mass, DM) and organic waste (0.15 g DM of either compost or manure) initially enriched with of a 14C-labelled antibiotic (14C-SMX: 0.74 mg.kg-1 DM; 14 C-CIP: 4.97 mg.kg-1 DM) were incubated under controlled aerated conditions for 28 days. The mineralization of the 14 C-antibiotic and the organic matter was followed over the incubation period. At 0, 7 and 28 days, the (bio)availability of each 14 C-antibiotic in mixtures was assessed through a soft chemical extraction with different aqueous solutions. A second extraction with an acetonitrile/water mixture was performed and solid residues were then dried and burnt in order to quantify the fraction of non-extractable 14 C-SMX and 14 C-CIP residues. The fractions of extracted 14 C-antibiotic residues differed according to the type of aqueous solution in both types of organic waste-soil mixtures. The availability of 14 C-SMX decreased rapidly over time. The availability of 14 C-CIP was very low at the start of the incubation but it slightly increased at 7 days for the manure treatment. Regardless of the choice of the aqueous solution, significant amounts of non- extractable residues (NER) were formed after 28 days. The choice of the aqueous solution impacted more the relative proportions of 14 C-antibiotic residues extracted by the aqueous solutions and the acetonitrile/water mixture than the amounts of NER. Less than 1% of the initially added 14 C-CIP was mineralized after 28 days, against 2-3% for 14 C-SMX. The mineralization of organic matter was more important with manure. We hypothesize that the stimulation of microbial activity may explain the greater formation of NER of 14 C-SMX residues for the manure treatment.
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Dates et versions

hal-01192537 , version 1 (02-09-2015)

Identifiants

  • HAL Id : hal-01192537 , version 1
  • PRODINRA : 287168

Citer

Anais Goulas, Valerie Bergheaud, Sabrina Ferhi, Valerie Dumeny, Sylvie Nelieu, et al.. Impacts of the origin and the treatment of organic waste recycled in agriculture on the fate and the availability of antibiotics in cultivated soils. European Chemistry Congress - 5 th EuCheMS, Aug 2014, Istanbul, Turkey. 565 p., 2014. ⟨hal-01192537⟩

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