Characterization of proteins secreted during maize microspore culture : arabinogalactan proteins (AGPs) stimulate embryo development - Archive ouverte HAL
Article Dans Une Revue European Journal of Cell Biology Année : 2004

Characterization of proteins secreted during maize microspore culture : arabinogalactan proteins (AGPs) stimulate embryo development

Résumé

To study molecules secreted from cultured plant cells that promote development, maize microspores were transferred into culture and the conditioned media were collected over time and analysed. Electrophoresis indicated that both non-glycosylated and glycosylated proteins including arabinogalactan proteins (AGPs) appeared in the medium and their concentration increased during the time of culture. The development of embryos was correlated with the presence of specific extracellular proteins, using an experimental system based on a tunicamycin inhibition test. In addition, a precise protein analysis was conducted using MALDI-TOF and ESI-MS-MS techniques. These approaches have allowed the identification of 5 other types of proteins: a cell wall invertase, two thaumatin isoforms, one 1-3 beta-glucanase and two chitinase isoforms. Altogether these experiments and results open ways for research aimed at understanding which molecules stimulate embryo formation. Moreover, AGPs may be used to stimulate the development of microspores (pollen embryogenesis) prepared from non-responsive genotypes.
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Dates et versions

hal-01190922 , version 1 (01-09-2015)

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Gisèle Borderies, Mickael Le Béchec, Michel Rossignol, Claude Lafitte, Erwan Le Deunff, et al.. Characterization of proteins secreted during maize microspore culture : arabinogalactan proteins (AGPs) stimulate embryo development. European Journal of Cell Biology, 2004, 83 (5), pp.205-212. ⟨10.1078/0171-9335-00378⟩. ⟨hal-01190922⟩
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