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Poster De Conférence Année : 2013

Tracking of the chemical modifications occuring during the dry heating of alpha-lactalbumin

Résumé

Dry heating is commonly used in food and pharmaceutical industries to decontaminate heat-sensitive samples such as egg white or therapeutic protein products. Under these conditions thermal stability of proteins is increased and the changes in protein structure are limited.However, it was noted that the functionalities of t he dry heated proteins are changed: for instance lysozyme, a poor foaming agent, gives stable foams after dry heating even if the secondary and tertiary structure of the protein are not affected. This discovery expends the use of dry heat treatment for functionalizing a wide variety of food proteins, even to those that resist wet-heat treatments of decontamination. A clear picture of dry-heat induced modifications of proteins is necessary especially for those dedicated to the formulation of infant formula. In this study, LC-MS/MS was used to track the chemical modifications on milk proteins as illustrated here with α-lactalbumin. The samplesadjusted to pH 6.5 and water activity a w= 0.23 were dry heated at 100°C for up to 24 hours. Dry heat treatment traps a fraction of α-lactalbumin molecules into non-native monomers.These non-native monomers were characterized by the loss of one or two water molecules per α-lactalbumin molecules. Using tandem mass spectrome tric peptide mapping, these chemical modification were attributed to i- the formation of pyroglutamic acid from the N-term glutamic acid and ii- the formation of an internal cyclic imide at position Asp64 . The structural and functional consequences of theses modifications are discussed.
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Dates et versions

hal-01190679 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-01190679 , version 1
  • PRODINRA : 211960

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Muhammad Gulzar, Said Bouhallab, Valérie Briard-Bion, Julien Jardin, Thomas Croguennec. Tracking of the chemical modifications occuring during the dry heating of alpha-lactalbumin. EUPA 2013, Oct 2013, Saint-Malo, France. , 2013. ⟨hal-01190679⟩
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