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Article Dans Une Revue FEBS Letters Année : 2013

The pentose catabolic pathway of the rice-blast fungus Magnaporthe oryzae involves a novel pentose reductase restricted to few fungal species

Résumé

A gene (MoPRD1), related to xylose reductases, was identified in Magnaporthe oryzae. Recombinant MoPRD1 displays its highest specific reductase activity toward L-arabinose and D-xylose. K-m and V-max values using L-arabinose and D-xylose are similar. MoPRD1 was highly overexpressed 2-8 h after transfer of mycelium to D-xylose or L-arabinose, compared to D-glucose. Therefore, we conclude that MoPDR1 is a novel pentose reductase, which combines the activities and expression patterns of fungal L-arabinose and D-xylose reductases. Phylogenetic analysis shows that PRD1 defines a novel family of pentose reductases related to fungal D-xylose reductases, but distinct from fungal L-arabinose reductases. The presence of PRD1, L-arabinose and D-xylose reductases encoding genes in a given species is variable and likely related to their life style. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Dates et versions

hal-01190564 , version 1 (01-09-2015)

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Sylvia Klaubauf, Cecile Ribot, Delphine Melayah, Arnaud Lagorce, Marc-Henri Lebrun, et al.. The pentose catabolic pathway of the rice-blast fungus Magnaporthe oryzae involves a novel pentose reductase restricted to few fungal species. FEBS Letters, 2013, 587 (9), pp.1346 - 1352. ⟨10.1016/j.febslet.2013.03.003⟩. ⟨hal-01190564⟩
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