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Article Dans Une Revue PLoS ONE Année : 2013

Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci

Résumé

We described a quorum-sensing mechanism in the streptococci genus involving a short hydrophobic peptide (SHP), which acts as a pheromone, and a transcriptional regulator belonging to the Rgg family. The shp/rgg genes, found in nearly all streptococcal genomes and in several copies in some, have been classified into three groups. We used a genetic approach to evaluate the functionality of the SHP/Rgg quorum-sensing mechanism, encoded by three selected shp/rgg loci, in pathogenic and non-pathogenic streptococci. We characterized the mature form of each SHP pheromone by mass-spectrometry. We produced synthetic peptides corresponding to these mature forms, and used them to study functional complementation and cross-talk between these different SHP/Rgg systems. We demonstrate that a SHP pheromone of one system can influence the activity of a different system. Interestingly, this does not seem to be dependent on the SHP/Rgg group and cross-talk between pathogenic and non-pathogenic streptococci is observed.
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Dates et versions

hal-01190550 , version 1 (29-05-2020)

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Betty Fleuchot, Alain Guillot, Christine Mezange, Colette Besset, Emilie Chambellon, et al.. Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci. PLoS ONE, 2013, 8 (6), ⟨10.1371/journal.pone.0066042⟩. ⟨hal-01190550⟩
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