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Article Dans Une Revue Bio-protocol Année : 2013

Neutral Comet Assay

Résumé

The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content. Briefly, cells are embedded in agarose, lysed and submitted to an electric field, before the staining step with a fluorescent DNA binding dye. Damaged DNA (charged DNA) migrates in this field, forming the tail of a “comet”, while undamaged DNA remained in the head of the “comet”. The following document describes the protocol to realize a neutral comet assay. This assay can be applied to different cell types and has been useful for numerous applications in fields of toxicology or DNA damage and repair.
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Dates et versions

hal-01188318 , version 1 (28-08-2015)

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Elisa Boutet-Robinet, Didier Trouche, Yvan Canitrot. Neutral Comet Assay. Bio-protocol , 2013, 3 (18), ⟨10.21769/BioProtoc.915⟩. ⟨hal-01188318⟩
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