High-NaCl perception in drosophila melanogaster - Archive ouverte HAL
Article Dans Une Revue Journal of Neuroscience Année : 2014

High-NaCl perception in drosophila melanogaster

Résumé

Salt is a fundamental nutrient that is required for many physiological processes, including electrolyte homeostasis and neuronal activity. In mammals and Drosophila, the detection of NaCl induces two different behaviors: low-salt concentrations provide an attractive stimulus, whereas high-salt concentrations are avoided. We identified the gene called serrano (sano) as being expressed in the sensory organs of Drosophila larvae. A transgenic reporter line showed that sano was coexpressed with Gr66a in a subset of gustatory neurons in the terminal organ of third-instar larvae. The disruption of sano gene expression in gustatory neurons led to the specific loss of high-salt concentration avoidance in larvae, whereas the detection of other attractive or aversive substances was unaffected. Moreover, using a cellular marker sensitive to calcium levels, Sano function was shown to be required for neuronal activity in response to high-salt concentrations. In these neurons, the loss of the DEG/ENaC channel PPK19 function also eliminated the cellular response to high-salt concentrations. Our study revealed that PPK19 and Sano are required in the neurons of the larval gustatory organs for the detection of high-salt concentrations.

Dates et versions

hal-01185022 , version 1 (18-08-2015)

Identifiants

Citer

Georges Alves, Jeremy Sallé, Sylvie Chaudy, Stéphane Dupas, Gérard Maniere. High-NaCl perception in drosophila melanogaster. Journal of Neuroscience, 2014, 34 (33), pp.10884 - 10891. ⟨10.1523/JNEUROSCI.4795-13.2014⟩. ⟨hal-01185022⟩
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