The current global issues have stimulated the search for both ecologically and economically friendly (eco?-friendly) materials and processes. As a sustainable and affordable biopolymer, cellulose is an ideal material for developing diagnostic devices. Recently, paper-based bioanalytical devices have trended towards three-dimensional microfluidic platforms allowing multiplex diagnosis. This technological mutation now challenges the production process of those devices. The whole design, as well as the biosensing material immobilization, should be as eco?-friendly as possible. To this end, the biomolecule immobilization process presented here combines a photolinker-free photografting procedure with inkjet printing which is a versatile and environmentally friendly dispensing method. While many printing cycles are usually achieved to get efficient immune answers, only one to five printing passes were sufficient in this study. Antibodies have been successfully printed and immobilized onto paper sheets. These membranes were further used to perform lateral flow immunoassays. The visual detection limits observed were identical to those usually displayed by the classical dispensing method, regardless the membrane material. The process developed herein is simple, time and cost-saving as well as environmentally friendly. More generally, it is a powerful tool for robust and abundant immobilization of chemical-sensitive proteins onto various cellulose-based papers and according to complex designs.