Article Dans Une Revue eLife Année : 2015

CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.

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The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

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hal-01138078 , version 1 (11-05-2015)

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Mayeul Collot, Christian D Wilms, Asma Bentkhayet, Païkan Marcaggi, Kiri Couchman, et al.. CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.. eLife, 2015, 4, pp.e05808. ⟨10.7554/eLife.05808⟩. ⟨hal-01138078⟩
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