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Article Dans Une Revue Archives of Virology Année : 2012

Encephalomyocarditis virus may use different pathways to initiateinfection of primary human cardiomyocytes

Résumé

Encephalomyocarditis virus (EMCV) caninfect a wide range of vertebrate species including swineand non-human primates, but few data are available forhumans. We therefore wanted to gain further insight intothe mechanisms involved in EMCV infection of humancells. For this purpose, we analyzed the permissiveness ofprimary human cardiomyocytes towards two strains ofEMCV; a pig myocardial strain (B279/95) and a rat strain(1086C). In this study, we show that both strains productivelyinfect primary human cardiomyocytes and inducecomplete cytolysis. Binding and infection inhibitionexperiments indicated that attachment and infection areindependent of sialic acid and heparan sulfate for B279/95and dependent for 1086C. Sequence comparison betweenthe two strains and three-dimensional analysis of the capsidrevealed that six of the seven variable residues are surfaceexposed,suggesting a role for these amino acids in binding.Moreover, analysis of variants isolated from the 1086Cstrain revealed the importance of lysine 231 of VP1 in theattachment of EMCV to cell-surface sialic acid residues.Together, these results show a potential for EMCV strainsto use at least two different binding possibilities to initiateinfection and provide new insights into the mechanismsinvolved in primary human cell recognition by EMCV.
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Dates et versions

hal-01137009 , version 1 (30-03-2015)
hal-01137009 , version 2 (03-11-2015)

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Saliha Hammoumi, Monique Guy, Marc Eloit, Labib Bakkali. Encephalomyocarditis virus may use different pathways to initiateinfection of primary human cardiomyocytes. Archives of Virology, 2012, 157, pp.43-52. ⟨10.1007/s00705-011-1133-6⟩. ⟨hal-01137009v2⟩
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