Effect of GA 3 and paclobutrazol on adventitious shoot regeneration of two Pelargonium sp - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2012

Effect of GA 3 and paclobutrazol on adventitious shoot regeneration of two Pelargonium sp

Résumé

This study had two aims. The first was to improve the regeneration efficiency of Pelargonium leaf discs by adventitious budding. The second was to test the effect of gibberellic acid (GA 3) and paclobutrazol (PBZ) on callus formation and adventitious shoot regeneration in Pelargonium before using genetic transformation of this species for functional validation of genes involved in the process of GA regulation. GA 3 and paclobutrazol (an inhibitor of GA synthesis pathway) were added (together or separately) in the shoot regeneration media of two Pelargonium species, Pelargonium * hortorum 'Panache sud' ('P.sud') and Pelargonium * domesticum 'Autumn haze' (' P.dom'). In both cases, GA 3 applied alone, completely inhibited the bud regeneration. Moreover, the rate of callus formation decreased drastically when 5 M of GA 3 was applied to ' P. dom' explants. Similar result was obtained with 'P.sud' explants using 20 M GA 3. Paclobutrazol (0.3 M) applied at the same time as GA 3 (10 M) could partially restore regeneration process of ' P. dom'. For ' P. dom', the use of paclobutrazol alone increased callus formation and slightly improved the rate of regeneration. Moreover, initiated buds had a better appearance. For 'P. sud', which had an abundant callusing, paclobutrazol did not improve regeneration and led to hyperhydric shoots.

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Dates et versions

hal-01132186 , version 1 (16-03-2015)

Identifiants

  • HAL Id : hal-01132186 , version 1
  • OKINA : ua8885

Citer

L. Hamama, L. Voisine, A. Naouar, R. Gala, D. Cesbron, et al.. Effect of GA 3 and paclobutrazol on adventitious shoot regeneration of two Pelargonium sp. Acta Horticulturae, 2012, Leuven, Belgium. pp.187-194. ⟨hal-01132186⟩
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