Pattern of expression of vascular endothelial growth factor and its receptors in the ovine choroid plexus during long and short photoperiods - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cell and Tissue Research Année : 2012

Pattern of expression of vascular endothelial growth factor and its receptors in the ovine choroid plexus during long and short photoperiods

Résumé

Vascular endothelial growth factor (VEGF-A) plays an important role in maintaining cerebrospinal fluid (CSF) homeostasis and the function of the choroid plexuses (CPs). The objective of the study was to determine the expression of vascular endothelial growth factor (VEGF-A), tyrosine kinase receptors Flt-1 and KDR and KDR co-receptor neuropilin 1 (NRP-1) in ovine CPs during different photoperiods. CPs were collected from the lateral brain ventricles from ovariectomized, estradiol-treated ewes during long day (LD; 16L:8D, n = 5) and short day (SD; 8L:16D, n = 5) photoperiods. We analyzed mRNA expression levels of two VEGF-A isoforms, VEGF-A (120) and VEGF-A (164) and our results indicate that VEGF-A (164) was the predominant isoform. Expression levels of VEGF-A and Flt-1 were similar during the SD and LD photoperiods. There were significant increases in KDR mRNA and protein expression (p < 0.05) and NRP-1 mRNA expression (p < 0.05) during SD. These data show that expression of KDR and its co-receptor NRP-1 are up-regulated by short photoperiod and that this effect is not dependent on ovarian steroids. Our results suggest that the VEGF-A-system may be involved in photoperiodic plasticity of CP capillaries and may therefore be responsible for photoperiodic changes in the CSF turnover rate in ewes.

Domaines

Autre [q-bio.OT]
Fichier principal
Vignette du fichier
2012_Szczepkowska_Cell and Tissue Research_1 (413.92 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01129667 , version 1 (29-05-2020)

Licence

Paternité

Identifiants

Citer

Aleksandra Szczepkowska, Barbara Wasowska, Przemyslaw D. Gilun, Christine Lagaraine, Vincent Robert, et al.. Pattern of expression of vascular endothelial growth factor and its receptors in the ovine choroid plexus during long and short photoperiods. Cell and Tissue Research, 2012, 350 (1), pp.157-166. ⟨10.1007/s00441-012-1431-7⟩. ⟨hal-01129667⟩
132 Consultations
28 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More