The hydrothermal synthesis of Mn-analcime was performed by mixing manganese(II) carbonate, silicic acid and aluminum nitrate in a basic medium in the 120–175 °C temperature range for 6–72 h. Different Mn/Al ratios were used. The obtained samples were characterized by powder XRD, N2 physical adsorption analysis, TGA/DTA, transmission electron microscopy, elemental analysis, IR, XPS, EPR and 29Si and 27Al MAS-NMR spectroscopies. Manganese is probably mostly in the +IV oxidation state and partly substitutes silicon in the zeolite framework. The catalytic properties of Mn-analcime were evaluated in the cyclohexene oxidation reaction and highlight an interesting activity of this material, together with a good recyclability, which make it a promising catalyst for selective oxidation reactions.